CD59 antibody (PE)

Catalog No. ABIN119474

The Rat Monoclonal anti-CD59 antibody has been validated for FACS. It is suitable to detect CD59 in samples from Human.

Quick Overview for CD59 antibody (PE) (ABIN119474)

Target: CD59

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This CD59 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: YTH53-1

Product Details anti-CD59 Antibody (PE)

Specificity: Clustered at the fourth leucocyte typing workshop to designation CD59. YTH53.1 reacts with CD59 transfectants. It enhances antibody-mediated lysis by homologous complement and reacts with GPI-linked antigen of 18-20 kDa size which is broadly expressed on human leucocytes and erythrocytes. Putative ligand for CD2.

Purification: Ion exchange chromatography

Immunogen: Human Peripheral Blood T cells. Spleen cells from immunised DA rat fused with the Y3/Ag1.2.3 meloma cell line.

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: We do not recommend using this antibody in lysed whole blood techniques due to inhibition of staining caused by CD59 expression on erythrocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for CD59

Target: CD59

Alternative Name: CD59

Background: CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. It is a potent inhibitor of the complement membrane attack complex action. It acts by binding to the C8 and/or C9 complements of the membrane attack complex, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is also involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.Synonyms: 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-IP, MAC-inhibitory protein, MACIF, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin

Gene ID: 966

NCBI Accession: NP_000602

UniProt: P13987

Pathways: Complement System